Preparation of a toxicity fusion protein targeting to CD123 on leukemia stem cell

VernacularTitle:靶向白血病干细胞CD123的毒性融合蛋白的制备
Author: Simei REN ; Yonjun ZHANG ; Hongwei PENG ; Jinhong WANG ; Qing JI ; Dongmei FAN ; Nan ZHANG ; Jie ZENG
Publication Type:Journal Article
Keywords: Fusion proteins; Interleukin 3; Tumor stem cell; Targeted therapy
From: Journal of Leukemia & Lymphoma 2011;20(8):490-493
CountryChina
Language:Chinese
Abstract: Objective To construct a fusion protein that used for treatment of resistance and palindromia in leukemia and studied its biological activity. Methods IL-3 and LP gene fragments were amplified by PCR. After enzymatic digestion and T4 ligation, the fusion gene was cloned into expression vector pAYZ. The product was purified by exchange chromatography and anti-Etag affinity chromatography. IL3-G4SLP fusion protein was analyzed by SDS-PAGE and Western blot. Protein biological activity was detected by FACS. Results The fusion protein was expressed as soluble protein by E.Coli 16C9. The protein expression level was about 1 mg/L, its purity was over 95 %, and the expression level was about 1 mg/L. The fusion protein can combined specificely with CD123 on leukemia stem cells. Conclusion Fusion protein IL-3-G4S-LP can target on leukemia stem cells and maybe as a potential drug used for treatment of resistance and palindromia in leukemia.