Corticotropin-releasing Factor (CRF) and Urocortin Promote the Survival of Cultured Cerebellar GABAergic Neurons Through the Type 1 CRF Receptor.
10.3346/jkms.2006.21.3.518
- Author:
Jae Sun CHOI
1
;
Thao Thi Hien PHAM
;
Yoon Jin JANG
;
Bao Chi BUI
;
Bong Hee LEE
;
Kyeong Min JOO
;
Choong Ik CHA
;
Kyung Hoon LEE
Author Information
1. Department of Anatomy, Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Korea. khlee@med.skku.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cerebellum;
Interneurons;
Corticotropin-Releasing Hormone;
urocortin;
Protective Agents
- MeSH:
gamma-Aminobutyric Acid/*metabolism;
Time Factors;
Receptors, Corticotropin-Releasing Hormone/*metabolism;
Peptides/chemistry;
Neurons/*metabolism;
Mice, Inbred C57BL;
Mice;
Immunohistochemistry;
Image Processing, Computer-Assisted;
Corticotropin-Releasing Hormone/biosynthesis/*physiology;
Cerebellum/*embryology/*metabolism;
Cells, Cultured;
Cell Survival;
Animals
- From:Journal of Korean Medical Science
2006;21(3):518-526
- CountryRepublic of Korea
- Language:English
-
Abstract:
Corticotropin releasing factor (CRF) is known to be involved in the stress response and in some degenerative brain disorders. In addition, CRF has a role as a neuromodulator in adult cerebellar circuits. Data from developmental studies suggest a putative role for CRF as a trophic factor during cerebellar development. In this study, we investigated the trophic role for CRF family of peptides by culturing cerebellar neurons in the presence of CRF, urocortin or urocortin II. Primary cell cultures of cerebella from embryonic day 18 mice were established, and cells were treated for either 1, 5 or 9 days with Basal Medium Eagles complete medium alone or complete medium with 1 micrometer CRF, urocortin, or urocortin II. The number of GABA-positive neurons in each treatment condition was counted at each culture age for monitoring the changes in neuronal survival. Treatment with 1 micrometer CRF or 1 micrometer urocortin increased the survival of GABAergic neurons at 6 days in vitro and 10 days in vitro, and this survival promoting effect was abolished by treatment with astressin in the presence of those peptides. Based on these data, we suggest that CRF or urocortin has a trophic role promoting the survival of cerebellar GABAergic neurons in cultures.
