Influence of Moxibustion Serum on the Expression of Fas bcl-2 mRNA and Protein of EL-4 Lymphoma Cells
10.1007/s11726-008-0331-6
- VernacularTitle:Influence of Moxibustion Serum on the Expression of Fas bcl-2 mRNA and Protein of EL-4 Lymphoma Cells
- Author:
Xiaopeng MA
;
Cuiying ZHAO
;
Yunfei CHEN
;
Huangan WU
;
Zheng SHI
;
Xian HONG
;
Ling YANG
- Publication Type:Journal Article
- Keywords:
Moxibustion Therapy;
Serum;
Neoplasms;
Fas Antigen;
Bcl-2 Protein,Mouse;
Gene Expression;
Mice
- From:
Journal of Acupuncture and Tuina Science
2008;6(6):331-333
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the influence of moxibustion sertma of mice on the expression of Fas, bcl-2 mRNA and protein of EL-4 lymphoma cells in vitro. Method: The EL-4 lymphoma cells were cultivated for 24 h in the moxibustion serum of mice. The expression of Fas and bcl-2 mRNA of EL-4 lymphoma cells were detected by in-situ hybridization method, and the protein expression of Fas and bcl-2 were observed by the immuocytochemistry method. Results: The expression of bcl-2 mRNA and protein decreased, and the expression of Fas rnRNA and protein increased significantly in EL-4 cells, which were cultivated in the moxibustion serum compared those cultivated in normal mice serum (P<0.05). Conclusion: Moxibustion serum could down-regulate the bcl-2 mRNA and protein and up-regulate the Fas mRNA and protein of EL-4 cells.
