Construction and identification of a recombinant Bifidobacteria (pGEX-TSO45W-4B) vaccine of Taenia solium

VernacularTitle:猪带绦虫重组双歧杆菌(pGEX-TSO45W-4B)疫苗构建及鉴定
Author: Biying ZHOU ; Meichen LIU ; Lifang HE
Publication Type:Journal Article
Keywords: Taeniasolium; Recombinant Bb(pGEX-TSO45W-4B) vaccine; Construction; Identification
From: Chinese Journal of Endemiology 2014;33(6):591-595
CountryChina
Language:Chinese
Abstract: Objective To construct and identify a recombinant Bifidobacteria(Bb) (pGEX-TSO45W-4B) vaccine of Taenia solium.Methods The TSO45W-4B gene of Taenia solium was synthesized.It was expected that the fragment length of the gene was 351 bp.The gene was cloned into Escherichia coli-Bifidobacteria(Bb) shutde vector pGEX-1λT to construct a recombinant plasmid pGEX-TSO45W-4B.The recombinant plasmid was electroporated into Bb to construct a recombinant Bb (pGEX-TSO45W-4B) vaccine.The vaccine was identified by restriction analysis,PCR and sequencing,which would demonstrate that the constructed recombinant Bb (pGEX-TSO45W-4B) vaccine of Taenia solium contained the gene of fragment length of 351 bp.Results The TSO45W-4B gene of 351 bp was synthesized.Restriction analysis,PCR and sequencing results showed that the recombinant Bb (pGEX-TSO45W-4B) vaccine of Taenia solium was successfully constructed and contained the gene fragment of 351 bp.Conclusion The recombinant Bb (pGEX-TSO45W-4B) vaccine of Taenia solium is successfully constructed,which lays a foundation for the study of expression and immunogenicity of the vaccine.