The effects of Xingnaojing injection on Caveolin-1 in cortex of brain after global ischemia-reperfusion
10.3760/cma.j.issn.1671-0282.2015.05.012
- VernacularTitle:醒脑静注射液对全脑缺血-再灌注大鼠脑内Caveolin-1表达的影响
- Author:
Junfeng CHEN
;
Bing LI
;
Ming LIU
;
Hongzhi LI
;
Xiaojun LIANG
;
Jie SHEN
;
Zhiyue ZHONG
- Publication Type:Journal Article
- Keywords:
Xingnaojing injection;
Cerebral ischemia hypoxia;
Brain;
Blood-brain barrie;
Caveolin-1;
Brain edema
- From:
Chinese Journal of Emergency Medicine
2015;24(5):501-505
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact of Xingnaojing (XNJ) injection (a preparation of Chinese herb medicine) on the permeability of blood-brain barrier and Caveolin-1 in cortex of brain after global ischemia-reperfusion.Methods Modified Pulsinelli method for four-vessel occlusion was employed to establish the global ischemia reperfusion model in rats.Male Wistar rats were randomly (random number) divided into three groups,namely sham group,model group and XNJ group.Each group was observed at 24 h,48 h and 72 h after ischemia reperfusion.The water content of brain tissue was determined by dry/wet weight ratio,while the Evans blue (EB) concentration in brain tissue was detected by spectrophotometer.Western blot was used to detect caveolin-1 level in the cerebral cortex.Results The water contents of brain tissue in model group and XNJ group were significantly higher than that in sham group 24 hour after ischemia-reperfusion (P ＜ 0.05).But at 48 h and 72 h after ischemia-reperfusion,the brain water content in model group was significantly higher than that in XNJ group and sham group (P ＜ 0.05).The EB concentrations in brain tissue in model group and XNJ group were higher than that in sham group 24 h after ischemia-reperfusion (P ＜ 0.05).EB levels in sham group and XNJ group were significantly lower than that in model group 48 h and 72 h after ischemia-reperfusion (P ＜ 0.05).Caveolin-1 levels in cerebral cortex in sham group and XNJ group were significantly higher than that in model group 24 h,48 h and 72 h after ischemia-reperfusion (P ＜ 0.05).Conclusions After global ischemia-reperfusion,Xingnaojing (XNJ) injection could protect blood-brain barrier in virtue of regulating caveolin-1 protein level.
