A comparison of fluorescence polarization assay with four classical serological methods in diagnosis of human brucellosis
10.3760/cma.j.issn.2095-4255.2015.06.019
- VernacularTitle:荧光偏振检测技术在布鲁杆菌病诊断中的对比实验分析
- Author:
Dongmei ZHAO
;
Zunrong ZHENG
;
Jingyao LIU
;
Huimei BI
;
Yuzhen ZHOU
;
Jingjing HE
;
Xun ZHOU
- Publication Type:Journal Article
- Keywords:
Fluorescence polarization assay;
Brucellosis;
The conventional serological methods
- From:
Chinese Journal of Endemiology
2015;34(6):459-461
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the diagnostic value of fluorescence polarization assay (FPA) for human brucellosis.Methods From April 2013 to August 2014,240 positive sera of brucella patients were collected as case group and 287 normal sera as control group in Heilongjiang Agricultural Reclamation Bureau General Hospital.Four milliliters of venous blood were withdrawn for separation of serum.Fluorescence polarization assay was used and the consistency,sensitivity and specificity were compared with those of four classical serological methods,including rose bengal plate agglutination test (RBPT),standard tube agglutination test (SAT),Coomb's test and cysteine test.Results The results of FPA were complied with those of the four conventional serological methods,Kappa ≥ 0.75.The sensitivity of FPA (89.17％) was higher than that of RBPT (87.08％),SAT (85.00％),Coomb's (74.17％) and cysteine test (75.83％).The specificity of FPA (94.08％) was higher than that of RBPT (89.90％) and Coomb's (81.53％),but lower than that of SAT (98.95％) and cysteine test (99.30％).Conclusion FPA can be used as a laboratory diagnostic method for human brucellosis,which is worthy to be widely used.
