Xinfukang drug-containing serum on bone marrow stem cell transcription and secretion of SDF-1α

VernacularTitle:心复康含药血清对骨髓干细胞转录和分泌SDF-1α的影响
Author: Bohong KAN ; Yijing WANG ; Xiantong HU ; Qing GAO ; Yingchang FAN ; Yingna HE ; Kongyin ZHAO
Publication Type:Journal Article
Keywords: Bone marrow stem cells; Xinfukang; SDF-1α
From: International Journal of Biomedical Engineering 2014;37(5):275-278
CountryChina
Language:Chinese
Abstract: Objective To clarify the effects of Xinfukang containing-serum on stromal cell-derived factor-1α (SDF-1α) translation and protein secretion of bone marrow stem cells (BMSCs).Methods BMSCs were isolated and amplified using bone marrow culture method,and were identified by flow cytometry.mRNA and protein secretion of SDF-1α were detected by quantitative PCR (q-PCR) and enzyme linked immunosorbent assay (ELISA),respectively.Results The expression of SDF-1α mRNA were significantly increased after 72 h in drug-containing serum,and SDF-1α mRNA in the experimental group was approximately 200 times as that in the control group (P＜0.05).Secretion of SDF-1 α in the experimental group (277.561 1 ± 15.651 8) pg/ml was nearly doubled compared with that in the control group (153.107 1±14.765 1) pg/ml (P＜0.05).Conclusions BMSCs from whole bone marrow adherent culture have high purity,and drug-containing serum can promote BMSCs to express SDF-1 α mRNA and secretion of SDF-1 α.