The establishment of human esophageal cancer model in Hu-PBL-SCID mice

VernacularTitle:免疫重建荷人食管癌SCID鼠肿瘤模型的建立
Author: Zhihong LU ; Minhong PANG ; Shanglin DONG ; Jing WANG ; Kaiyang LIU
Publication Type:Journal Article
Keywords: SCID mouse; Esophageal neoplasms; Animal models
From: Cancer Research and Clinic 2015;27(6):381-384
CountryChina
Language:Chinese
Abstract: Objective To establish a mouse model for human esophageal cancer.Methods Human PBL were isolated directly from whole blood by density gradient centrifugation.Fifteen SCID mice were randomly divided into two groups.Group A was control group,and in group B there were 12 mice intraperitoneally injected with 2×107 human PBL and subcutaneously injected with 5×106 ECA109 cells.The rate of tumor transplantation,tumor growth,metastasis and histological features were observed.After 3,4,5,6 weeks of engraftment,the level of IgG in mouse serum and the spleen weight were detected.Results The successful rate of tumor transplantation was 100 ％.Metastasis was not found.After 3,4,5,6 weeks of engraftment,the spleen weight in group B were (55.44±4.45) mg,(88.62±2.24) mg,[(125.98±2.19) mg] (P ＜ 0.05) and (213.71±2.96) mg,which had statistical significance compared with the control group (41.87±2.97) mg.The level of IgG was significantly higher than that in control group (P ＜ 0.01).Conclusion The experimental results demonstrate that human esophageal cancer models have been established in Hu-PBL-SCID mice.