Application of rapid detection of MRSA ST239 clones in bloodstream infections
10.3760/cma.j.issn.1009-9158.2015.01.011
- VernacularTitle:血流感染MRSA中的ST239克隆株快速检测与分析
- Author:
Jie YAO
;
Kai YANG
;
Zhou LIU
;
Liwen CHEN
;
Qiang ZHOU
;
Shihe GUAN
- Publication Type:Journal Article
- Keywords:
Sepsis;
Methicillin resistance;
Staphylococcus aureus;
Staphylococcal infections;
Clone cells;
Bacteriological techniques
- From:
Chinese Journal of Laboratory Medicine
2015;38(1):40-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluation the method of rapid detection of Methicillin-resistant Stphylococcus aureus (MRSA) ST239 clones with multiplex PCR assay and investigation of the epidemic status of MRSA blood stream infections in Hefei area.Methods Antibiotic susceptibility testing were applied to MRSA isolates from bloodstream infection,rapid screening and confirmation of MRSA ST239 clones by using multiplex PCR,Multilocus Sequence typing (MLST) and Staphyloccoccal Cassette Chromosome mec(SCCmec) typing.Results 51 of 106 clinic isolates Staphylococcus aureus were identified as MRSA,accounting for 48.1％.The resistance rate of MRSA to erythromycin,aminoglycosides and quinolone were significantly higher than Methicillin Sensitive Staphylococcus aureus (MSSA).Both MRSA and MSSA had a high sensitivity to cotrimoxazole,the sensivity rates were 86.3％ and 94.5％,respectively; 47 of 51 isolates of MRSA that detected by MRSA ST239 rapid screening were ST239 clones.Randomly selected 20 positive screening stains were confirmed as MRSA-ST239-SCCmec Ⅲ by MLST and SCCmectyping.Conclusions In Hefei area,nearly half of MRSA bloodstream infections in clinical isolates are MRSA-ST239-SCCmeclⅢ type and serious multidrug-resistance.The rapid detection of ST239 clones by multiplex PCR is a reliable and effective method for large-scale screening in laboratory.
