Effect of exogenous hydrogen sulfide on myocardial damage induced by renal ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2014.12.019
- VernacularTitle:外源性硫化氢对大鼠肾缺血再灌注诱发心肌损伤的影响
- Author:
Jing DU
;
Bing ZHANG
;
Hongyan DAI
;
Wei FENG
;
Sen HUANG
;
Qin ZHANG
;
Wei XIE
- Publication Type:Journal Article
- Keywords:
Hydrogen sulfide;
Reperfusion injury;
Kidney;
Myocardium
- From:
Chinese Journal of Anesthesiology
2014;34(12):1478-1480
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of exogenous hydrogen sulfide (H2 S) on myocardial damage induced by renal ischemia-reperfusion (I/R) in rats.Methods Thirty pathogen-free male Wistar rats,weighing 250-300 g,were randomized into 3 groups (n =10 each) using a random number table:sham operation group (S group); myocardial damage induced by renal I/R group (I/R group); NaHS group.Renal I/R was induced by occlusion of the left kidney for 120 min followed by 60 min reperfusion in anesthetized rats.In NaHS group,NaHS 100 μg/kg was injected intraperitoneally at 20 min before reperfusion.At the end of reperfusion,the rats were sacrificed and the hearts were obtained for determination of malondialdehyde (MDA) content and glutathione peroxidase (GSH-Px) activity in myocardial tissues and for microscopic examination.Results Compared with S group,MDA content was significantly increased,and GSH-Px activity was decreased in I/R and NaHS groups.Compared with I/R group,MDA content was significantly decreased,and GSH-Px activity was increased in NaHS group.The pathological changes of myocardium were significantly attenuated in NaHS group as compared with I/R group.Conclusion Exogenous H2 S can alleviate myocardial damage induced by renal I/R in rats.
