Role of Src tyrosine kinase in damage to alveolar epithelial cells caused by mechanical stretch
10.3760/cma.j.issn.0254-1416.2014.12.006
- VernacularTitle:Src激酶在机械通气性牵张致肺泡上皮细胞损伤中的作用
- Author:
Tao ZHAO
;
Mengjie LIU
;
Changping GU
;
Yuelan WANG
- Publication Type:Journal Article
- Keywords:
Src-family kinases;
Biomechanics;
Epithelial cells
- From:
Chinese Journal of Anesthesiology
2014;34(12):1436-1438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of Src tyrosine kinase in damage to alveolar epithelial cells caused by mechanical stretch.Methods MLE-12 cells cultured in vitro were randomly divided into 3 groups using a random number table:mechanical stretch group (group S),dimethyl sulfoxide control group (group D),and Src tyrosine kinase inhibitor PP2 group (group P).In D and P groups,dimethyl sulfoxide 30 μl/ml and PP2 100 μmol/L were added to the culture medium,respectively,and the cells were then cultured for 30 min.The cells underwent mechanical stretch for 8 h with frequency of0.5 Hz and amplitude of 20％ in the three groups.At 0,2,4 and 8 h of mechanical stretch,MLE-12 cells in 3 wells of each group were collected for determination of cell apoptosis with flow cytometry and expression of occludin using Western blot.The apoptosis rate was calculated.Results Compared with S group,no significant changes were found in the apoptosis rate and expression of occludin at each time point in group D,and the apoptosis rate was significantly decreased,and the expression of occludin was up-regulated at 2,4 and 8 h of mechanical stretch in group P.Conclusion The activation of Src tyrosine kinase is involved in damage to alveolar epithelial cells caused by mechanical stretch.
