Effect of penehyclidine hydrochloride on endotoxemia-induced intestinal injury in neonatal rats
10.3760/cma.j.issn.0254-1416.2015.02.027
- VernacularTitle:盐酸戊乙奎醚对内毒素血症新生大鼠肠损伤的影响
- Author:
Lihua JIANG
;
Lijuan WANG
;
Junbo ZHAO
;
Xiaoyong WEI
;
Tao WANG
;
Li LI
;
Bo LIU
- Publication Type:Journal Article
- Keywords:
Cholinergic antagonists;
Endotoxemia;
Intestine;
Infant,newborn
- From:
Chinese Journal of Anesthesiology
2015;35(2):242-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of penehyclidine hydrochloride on endotoxemiainduced intestinal injury in neonatal rats.Methods Ninety healthy neonatal Sprague-Dawley rats,aged 7days,weighing 16-20 g,were randomly divided into 3 groups (n =30 each) using a random number table:control group (group C),lipopolysaccharide (LPS) group,and penehyclidine hydrochloride group (group P).Endotoxemia was induced by intraperitoneal LPS 5 mg/kg in LPS and P groups.In group P,penehyclidine 2 mg/kg was injected intraperitoneally at 30 min before and after LPS injection.The equal volume of normal saline was given in group C.At 2,6 and 12 h after LPS or normal saline administration,10 rats in each group were randomly chosen and sacrificed.The ileum was removed to detect wet/dry lung weight ratio (W/D ratio) and contents of tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),hypoxia-inducible factor 1 alpha (HIF-1α) and glutamine (Gln),and for examination of the pathologic changes of the ileum with light microscope.Results Compared with group C,the W/D ratio and contents of TNF-α,IL-6 and HIF-1α were significantly increased,and the content of Gln was decreased at each time point in LPS and P groups.Compared with group LPS,the W/D ratio and contents of TNF-α,IL-6 and HIF-1α were significantly decreased,the content of Gin was increased at each time point,and the pathologic changes of the ileum were mitigated in group P.Conclusion Penehyclidine hydrochloride can reduce endotoxemia-induced intestinal injury in the neonatal rats,and down-regulated expression of HIF-1α,up-regulated expression of Gln and attenuated inflammatory responses may be involved in the mechanism.
