Effect of sepsis on vecuronium-induced inhibition of acetylcholine release in neuromuscular junction in rats
10.3760/cma.j.issn.0254-1416.2015.02.011
- VernacularTitle:脓毒症因素对维库溴铵抑制大鼠神经肌肉接头乙酰胆碱释放的影响
- Author:
Yujia WU
;
Feng GAO
;
Cong YU
;
Guijin HUANG
;
Ying YAO
;
Sisi LI
- Publication Type:Journal Article
- Keywords:
Sepsis;
Acetylcholine;
Neuromuscular nondepolarizing agents
- From:
Chinese Journal of Anesthesiology
2015;35(2):181-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sepsis on vecuronium-induced inhibition of acetylcholine release in neuromuscular junction in rats.Methods Thirty-six adult male SPF SpragueDawley rats,aged 2-3 months,weighing 200-220 g,were randomly divided into 3 groups (n=12 each) using a random number table:control group (group C),sham operation group (group S) and sepsis group (group Sep).Sepsis was induced by cecum ligation and puncture (CLP) in rats anesthetized with intraperitoneal chloral hydrate 350 mg/kg.At 12 h after CLP,the sciatic nerve-pretibial muscle was prepared.Vecuronium was added to the culture medium with the final concentration of 0.08 μg/ml,and the sciatic nerve-pretibial muscle was incubated for 15 min.Before and after administration,evoked endplate potentials (EPPs) and miniature endplate potentiais (MEPPs) were recorded by using intracellular microelectrode.EPP/MEPP ratio was calculated.Results Compared to C and S groups,EPPs,MEPPs and EPP/MEPP ratio were significantly increased before and after administration in group Sep.EPPs,MEPPs and EPP/MEPP ratio were significantly lower after administration than before administration in the three groups.Conclusion Sepsis can promote acetylcholine release in neuromuscular junction,thus weakening vecuronium-induced inhibition of acetylcholine release in neuromuscular junction in rats.
