Role of necroptosis in intestinal ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2014.12.016
- VernacularTitle:程序性坏死在大鼠肠缺血再灌注损伤中的作用
- Author:
Wenjing YANG
;
Shihong WEN
;
Yihong LING
;
Jiaxin LIU
;
Jiantong SHEN
;
Yunsheng LI
;
Kexuan LIU
- Publication Type:Journal Article
- Keywords:
Intestines;
Reperfusion injury;
Cell death
- From:
Chinese Journal of Anesthesiology
2014;34(12):1468-1470
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of necroptosis in intestinal ischemia-reperfusion (I/R) injury in rats.Methods Thirty-two healthy male Sprague-Dawley rats,weighing 200-220 g,were randomly assigned into 4 groups (n =.8 each) using a random number table:sham operation group (Sham group),I/R group,necroptosis inhibitor necrostatin-1 group (Nec-1 group) and solvent dimethyl sulfoxide (DMSO) group (group DMSO).Intestinal I/R injury was produced by clamping the superior mesenteric artery for 1 h followed by 24 h reperfusion in rats anesthetized with chloral hydrate.Necrostatin-1 1.0 mg/kg was administered intraperitoneally at 30 min before occlusion in Nec-1 group,while the equal volume of DMSO was given instead in group DMSO.The rats were sacrificed at 24 h of reperfusion and the intestinal tissues were removed for microscopic examination.Intestinal damage was assessed and scored according to Chiu.Blood samples were taken for determination of serum diamine oxidase (DAO) activity.The expression of activitied caspase-3 and receptor-interacting protein 1 (RIP1) in intestinal tissues was detected using Western blot.Results Compared with Sham group,Chiu's score,serum DAO activity,and the expression of activitied caspase-3 and RIP1 was up-regulated in I/R,DMSO and Nec-1 groups.Compared with I/R and DMSO groups,Chiu's score and DAO activity were significantly decreased,the expression of RIP1 was down-regulated,and no significant change was found in the expression of activitied caspase3 in group Nec-1.Conclusion Necroptosis is involved in intestinal I/R injury in rats.
