Effects of octanol on MMP-9 and TIMP-1 protein expression and brain edema after ischemia reperfusion in rats
10.3760/cma.j.issn.1674-6554.2015.02.004
- VernacularTitle:辛醇对大鼠脑缺血再灌注后基质金属蛋白酶-9组织型金属蛋白酶抑制剂-1表达及脑水肿的影响
- Author:
Meijuan YAN
;
Liao WU
;
Yanbo CHENG
;
Deqin GENG
;
Pengcheng XU
;
Ruiguo DONG
- Publication Type:Journal Article
- Keywords:
Octanol;
Reperfusion injury;
Brain edema;
Matrix metalloproteinase-9;
Tissue inhibitor of metalloproteinase-1
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2015;24(2):109-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the treatment of octanol on matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) protein expression,cerebral water content,infarction volume after ischemia-reperfusion in rats.Methods 150 SD rats were randomly divided into sham operated group (n=24),MCAO group (n=24),DMSO solvent control group (n=24) and octanol treatment group (n=24).A model of middle cerebral artery occlusion was induced by suture method.TTC stain was used to detect the infarction volume,dry-wet weight method to determine the brain water content.The expression of MMP-9 and TIMP-1 protein was detected by immunofiuorescence and Western blot.Results At 24 h of reperfusion after ischemia for 2 h,the octanol treatment group compared with MCAO group brain infarction volume obviously decreased(P＜0.05),water content significantly reduced ((78.16± 1.47) ％ vs (80.88±0.73) ％,P＜0.05),the number of MMP-9 positive cells obviously decreased((10.67±2.16) vs (29.00±3.40),P＜0.05),the expression of MMP-9 protein significantly reduced ((0.14±0.01) vs (0.21±0.02),P＜0.05)and the number of TIMP-1 positive cells significantly increased ((27.83 ±2.13) vs (5.67± 1.03),P＜0.05),the expression of TIMP-1 protein obviously increased((0.42±0.01) vs (0.28± 0.01),P＜0.05).The difference between MCAO group and DMSO solvent control group was not statistically significant(P ＜0.05).Conclusion Octanol may reduce brain edema,brain infarction volume.Up-regulation the expression of MMP-9 and down-regulation the expression of TIMP-1 may be one of the underlying mechanisms of the octanol neuroprotection.
