bFGF involved in the protection effect of radiation-induced apoptosis of neural stem cells by modulate the expression of Notch signal system protein
10.3760/cma.j.issn.1674-6554.2014.10.004
- VernacularTitle:碱性成纤维细胞生长因子调节Notch信号通路下游蛋白参与神经干细胞抗辐射损伤的实验研究
- Author:
Jing SU
;
Songhua XIAO
;
Jun LIU
- Publication Type:Journal Article
- Keywords:
Hes1 protein;
RBP-Jk protein;
Basic fibroblast growth factor;
Radiation injury
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2014;23(10):875-877
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of exogenous basic fibroblast growth factor (bFGF) on radiation-induced apoptosis of C17.2 neural stem cells(NSCs) and explore the relationship between bFGF and Hes1,RBP-JK protein.Methods The cell viability was assessed using the MTT assay.After the cells attach to the flasks they were irradiated by the linear accelerator.And 5 min later,different concentrations of bFGF in accordance with the experimental design and cultured cells 48 h.Extracted total protein of each group and Western blot analysis showed the changes of the Hes1,RBP-JK protein.Results Compared with the control group,irradiation group cell growth was inhibited,the OD of 0 ng/ml bFGF group was 0.61±0.81,the OD of 80 ng/ml bFGF group was 1.21±1.01 and the control group was 1.51± 1.13.Compared with the control group,all groups showed statistically significant difference (P＜ 0.05).Western blot analysis showed that with the increasing concentrations of bFGF,RBP-JK expression gradually decreased,but Hes1 expression gradually increased.Conclusion Exogenous basic fibroblast growth factor (bFGF) can inhibit apoptosis of C17.2 NSCs.bFGF can regulate Notch signaling pathway downstream proteins Hes1,RBP-JK expression and inhibit neural stem cell apoptosis which were irradiated.
