Changes of the level of phosphorelated ERK1/2 in the spinal trigeminal nucleus of migraine model rats
10.3760/cma.j.issn.1674-6554.2014.09.004
- VernacularTitle:偏头痛模型大鼠三叉神经脊束核磷酸化ERK1/2水平变化
- Author:
Xinquan JIANG
;
Lili SUN
;
Na MA
;
Tuo WU
;
Yuan NING
;
Yanbo ZHANG
;
Jiguo ZHANG
- Publication Type:Journal Article
- Keywords:
Migraine;
ERK1/2;
Central sensitization;
Spinal trigeminal nucleus;
Phosphorylation
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2014;23(9):781-783
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of ERK1/2 in the central pathogenesis of migraine.Methods Healthy adult male SD rats were randomly divided into five groups:normal group (group C),sham operation group(group C),migraine model group(group M),DMSO group (group D)and PD-98059group (PD group),with 12 rats in each group.The extracellular discharge frequency in the spinal trigeminal nucleus was recorded and ERK1/2 phosphorylation was tested.Results (1) The percentage of extracellular discharge frequency change:Two hours after treatment,the percentage of discharge frequency change was (325.9±47.32)％.The percentage of extracellula discharge frequency change in group M (325.9±47.3)％ was higher than that in group N (100.0± 0.0) ％ and group C(107.3± 16.4)％.There was no significant difference in the percentage of discharge frequency change between group D(319.3±42.5) ％ and group M (325.9±47.3) ％.The percentage of discharge frequency change in group PD(218.5±31.7)％ was lower than that in group M(325.9±47.3)％ and group D(319.3± 42.5)％.(2) ERK1/2 phosphorylation:the ERK1/2 phosphorylation in group M and group D was higher than that in group N and group C.There was no significant difference in ERK1/2 phosphorylation between group D and group M.The ERK1/2 phosphorylation in group PD was lower than the other four groups.Conclusion During the process of central sensitization to migraine,neuronal excitability and ERK1/2 phosphorylation were increased.ERK1/2 inhibitor (PD98059) reduced ERK1/2 phosphorylation and neuronal excitability.These indicated that ERK1/2 may play a role in central sensitization of migraine in rats.
