Protective effects of crocin in oxidative stress cell model and the effect on PI3K/Akt signal pathway

VernacularTitle:藏花素对氧化应激细胞模型的保护作用及PI3K/Akt通路的影响
Author: Yong CUI ; Yanjie WANG ; Danyu ZHAO ; Xiaofan FENG ; Haining GAO ; Xiaojing YANG ; Chun LIU
Publication Type:Journal Article
Keywords: Crocin; Oxidative stress; Apoptosis; PI3K/AKT
From: Chinese Journal of Behavioral Medicine and Brain Science 2015;24(3):215-217
CountryChina
Language:Chinese
Abstract: Objective To investigate the protective effect of crocin on oxidative stress cell model of PC 12 cell and the effect of crocin on PI3K/Akt signal pathway,as well as further explore the mechanism of protective effect on model cells.Methods Cells were divided into control group,model group,crocin group and VE group.The cell survival rate was detected by MTT method,and the expression of mRNA and protein of PI3K/Akt were detected by RT-PCR and Western blot.Results With the crocin concentration in 0.625 μM and 5 μM,the cell survival rate increased in a dose-dependent manner.The average optical density rate of PI3K and Akt mRNA were 0.435±0.044 and 0.375 ± 0.034,and the PI3K and Akt protein were 0.378± 0.038 and 0.386± 0.043 of crocin group.Compared with the model group,the expression levels of PI3K/Akt increased in crocin group (P＜0.05).Conclusion These results indicate that the antioxidant and antiapoptosis effects of crocin are induced via increasing expression of PI3K and pAkt.