Association study on tryptophan hydroxylase 2 promoter region gene polymorphisms with alcohol dependence
10.3760/cma.j.issn.1674-6554.2015.02.007
- VernacularTitle:色氨酸羟化酶2启动子区基因多态性与酒依赖的关联性研究
- Author:
Qiufeng LI
;
Chunling TANG
;
Jian HU
- Publication Type:Journal Article
- Keywords:
Alcohol dependence;
Tryptophan hydroxylase 2;
Gene polymorphisms
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2015;24(2):120-123
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association of single nucleotide polymorphisms (SNPs) of loci rs4570625,rs11178997 at promoter region of tryptophan hydroxylase 2 (TPH2) genes with alcohol dependence and depressive symptom in the alcohol-dependent subjects in north Chinese Han people.Methods Genotype and allele frequencies of the TPH2 rs4570625 and rs1 1178997 polymorphisms were examined in 60 alcohol-dependent subjects (patient group) and 62 normal controls (control group) by Polymerase Chain Reaction (PCR) amplification and DNA sequencing techniques.The alcohol-dependent subjects were evaluated by 24 Hamilton Depression Scale (HAMD).Association analysis was carried out between the polymorphism and symptom of depression in the alcohol-dependent subjects.Results There were significantly statistical differences in the rs4570625 alleles (G and T) (x2 =5.280,P＜0.05),but no statistically significant differences in the genotypes (G/G,G/T,T/T) (x2 =5.078,P＞0.05) between patient group and control group.Significant statistically difference could be found in the rs4570625 alleles (G and T) between the alcohol-dependent subjects who had depressive symptoms and the subjects who did not have depressive symptoms (P＜0.05),but no significantly statistical differences could be found in the genotypes frequency (P＞0.05).No significant statistically differences in the genotype and allele frequencies of the rs 11178997 were observed between the alcohol-dependent subjects and normal controls and the alcohol-dependent subjects who had depressive symptoms and the subjects who did not have depressive symptoms (all P＞0.05).Conclusion The results suggest that the liability of alcohol dependence and depressive symptoms in alcohol-dependent subjects may be associated with the polymorphisms of TPH2 rs4570625 in north Chinese Han population,and the variant of the T allele may contribute to the morbidity.However,the polymorphisms of TPH2 rs1 1178997 may have no association with the susceptibility of alcohol dependence and the depressive symptoms accompanied with alcohol dependence.
