Effect of insulin on the expression of human urate transporter gene in renal proximal tubule epithelial HK-2 cells
10.3760/cma.j.issn.1000-6699.2015.03.020
- VernacularTitle:胰岛素对肾近端小管上皮HK-2细胞人尿酸转运子基因表达的影响
- Author:
Wei SONG
;
Kun ZHANG
;
Changgui LI
;
Ying CHEN
;
Zhimin MIAO
- Publication Type:Journal Article
- Keywords:
Insulin;
Hyperuricemia;
Human urate transporter
- From:
Chinese Journal of Endocrinology and Metabolism
2015;31(3):282-284
- CountryChina
- Language:Chinese
-
Abstract:
Human proximal tubule epithelial cell line,HK-2 cells,were cultured with various concentrations of insulin for 48 h.Human urate transporter (hUAT) mRNA was detected by realtime quantitative PCR.hUAT mRNA levels were down-regulated by insulin (5,25,125,500 μIU/ml)in a dose-dependent manner (relative expression median were 0.95,0.40,0.24,and 0.23).In vitro,the expression of hUAT mRNA in HK-2 cells is associated with the concentration of insulin.
