Pathogeny of an epidemic of Vibrio cholerae in Jinzhou of Hubei Province in 2012

VernacularTitle:2012年湖北省荆州地区一起霍乱弧菌疫情分离株的病原学研究
Author: Ting ZHANG ; Hongmei YANG ; Ting CHENG ; Guoming LI
Publication Type:Journal Article
Keywords: Vibrio cholerae; Virulence gene; Pulse field gel electrophoresis
From: Chinese Journal of Endemiology 2015;34(6):421-424
CountryChina
Language:Chinese
Abstract: Objective To test the pathogeny of Vibrio cholerae strains of the epidemic in Jinzhou of Hubei Province.Methods Traditional methods of biochemistry,immobihzation test,string test and typing of blood serum were used to test the 6 strains isolated.The virulence gene which was cholera enterotoxin (ct) was detected by PCR.The whole genome DNA finger print of confirmed strains was analyzed by pulsed field gel electrophoresis (PFGE) after digestion respectively with two enzymes Not Ⅰ and Sfi Ⅰ.The DNA fingerprints were analyzed for clusters.Results The 6 strains of Vibrio cholerae were all O139 by traditional laboratory tests;virulence gene was detected in all 6 strains.The banding pattern was the same in the two maps of PFGE.The results of cluster analysis showed that the similarity coefficient of the six strains was 100％.Conclusion The epidemic of Vibrio cholerae is caused by the same pathogenic bacterium which is O139.