Expression changes of matrix metalloproteinases and tissue inhibitor of metalloproteinases in the articular cartilage of patients with Kashin-Beck disease
10.3760/cma.j.issn.2095-4255.2015.05.009
- VernacularTitle:大骨节病患者关节软骨基质金属蛋白酶和基质金属蛋白酶组织抑制剂的变化
- Author:
Jing MA
;
Xiong GUO
;
Xiaowei SHI
;
Shixun WU
;
Zengtie ZHANG
;
Qiang ZHANG
- Publication Type:Journal Article
- Keywords:
Kashin-Beck disease;
Matrix metalloproteinase-1;
Matrix metalloproteinase-3;
Tissue inhibitor of metallo proteinase-3;
Immuohistochemistry
- From:
Chinese Journal of Endemiology
2015;34(5):344-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in the expression of matrix metalloproteinase-1 (MMP-1),matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-3 (TIMP-3) in the articular cartilage of patients with Kashin-Beck disease (KBD) and the role of these proteins in pathogenesis of KBD.Methods The postoperative cartilage samples were collected from patients with KBD,osteoarthritis and patients with non-bone disease (control).The expression of MMP-1,MMP-3 and TIMP-3 in the cartilage was detected by immuohistochemistry,and the positive chondrocytes were counted in different layers of the articular cartilage under microscope.Results The positive rates of MMP-1 in the upper [(67.00 ± 1.69)％] and deeper [(22.07 ± 29.66)％] layers of articular cartilage from patients with KBD,and in the deeper layer of articular cartilage from patients with osteoarthritis [(70.52 ± 37.84)％] were significantly higher than those in the control group [(51.73 ± 36.74)％,(3.75 ± 6.85)％,all P ＜ 0.05].The positive rates of MMP-3 in the deeper layer of articular cartilage from patients with KBD [(28.84 ± 31.13)％] and in the middle and deeper layers of articular cartilage from patients with osteoarthritis [(55.69 ± 35.00)％,(45.96 ± 35.38％)] were significantly higher than those in normal cartilage [(34.09 ± 28.54)％,(14.46 ± 18.32)％,all P ＜ 0.05].The positive rates of TIMP-3 in the middle layer of articular cartilage from patients with KBD [(21.25 ± 25.23)％] and in the middle and deeper layers of articular cartilage from patients with osteoarthritis [(20.40 ± 22.19)％,(18.10 ± 22.58)％] were significantly lower than those in normal cartilage [(36.74 ± 26.61)％,(7.81 ± 20.58)％,all P ＜ 0.05].Conclusion MMP-1,MMP-3 and TIMP-3 play important roles in the articular cartilage damage of KBD.
