Construction and identification of recombinant vaccine Bifidobacterium bifidum (pGEX-Sj32) of Schistosoma japonicum
10.3760/cma.j.issn.2095-4255.2015.01.010
- VernacularTitle:日本血吸虫重组两歧双歧杆菌(pGEX-Sj32)疫苗构建及鉴定
- Author:
Jianrong TAN
;
Wengui LI
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Recombination,genetic;
Bifidobacterium bifidum;
Vaccines
- From:
Chinese Journal of Endemiology
2015;34(1):37-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct and identify recombinant vaccine Bifidobacterium bifidum (pGEX-Sj32) of Schistosomajaponicum (Sj).Methods The Sj32 gene amplified by PCR from template of plasmid pET28α-Sj32 that extracted from recombinant Escherichia coli(E.coli) BL21 (pET28α-Sj32) stored in our laboratory was cloned into E.coli-Bifidobacterium bifidum shuttle expression vector pGEX-1λT to construct recombinant plasmid pGEX-Sj32.The recombinant plasmid was transformed into E.coli BL21 (DE3).The plasmid was extracted and identified by restriction enzyme digestion.The recombinant Bifibacterium bifidum (pGEX-Sj32) vaccine was constructed by electroporating pGEX-Sj32 into Bb.The extracted plasmid was amplified and identified by PCR.Results The gene Sj32 of 1 270 bp in length was amplified by PCR.The restriction endonuclease digestion showed that the length of plasmid vector was 4 947 bp,and Sj32 gene was 1 270 bp.The Sj32 gene amplified by PCR from the template of pGEX-Sj32 extracted from recombinant Bb (pGEX-Sj32) vaccine was 1 270 bp in length which consistent with expected result.Conclusion The recombinant Bb (pGEX-Sj32) vaccine of Sj is successfully constructed.
