Effect of advanced age on sepsis-caused heterogeneity of nicotinic acetylcholine receptors in cytomembrane of skeleton muscle in rats
10.3760/cma.j.issn.0254-1416.2014.10.012
- VernacularTitle:老龄因素对脓毒症诱发大鼠骨骼肌细胞膜烟碱型乙酰胆碱受体异质化的影响
- Author:
Fei XIE
;
Su MIN
;
Li LIU
;
Xuechao HAO
;
Xianlin ZHU
- Publication Type:Journal Article
- Keywords:
Age factors;
Aged;
Sepsis;
Receptors,cholinergic;
Genetic heterogeneity
- From:
Chinese Journal of Anesthesiology
2014;34(10):1201-1203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of advanced age on sepsis-caused heterogeneity of nicotinic acetylcholine receptors (nAChR) in the cytomembrane of skeleton muscle in rats.Methods Twenty SPF adult rats (aged 4-5 months,weighing 250-280 g) and 20 aged male Sprague-Dawley rats (aged 18-20 months,weighing 550-600 g),were obtained from the Experimental Animal Centre of Chongqing Medical University.The adult rats were randomly divided into control group (CAd group,n =10) and sepsis group (SAd group,n =10).The aged rats were randomly divided into control group (CAg group,n =10) and sepsis group (SAg group,n =10).Sepsis was produced by cecal ligation and puncture.The specimens of anterior tibial muscle were obtained at 24 h after operation for determination of the expression of neuronal nAChR (α7-nAChR) and fetal nAChR (γ-nAChR) using immunohistochemistry and Western blot.Results The expression of γ-nAChR and α7-nAChR in the cytomembrane of anterior tibial muscle was significantly higher in CAg and SAd groups than in CAd group,and in SAg group than in CAg and SAd groups.Conclusion Advanced age can aggravate sepsis-induced heterogeneity of nAChR in the cytomembrane of skeleton muscle in rats.
