Effects of propofol on apoptosis and invasiveness of human lung cancer cell line A549 cells
10.3760/cma.j.issn.0254-1416.2014.09.013
- VernacularTitle:异丙酚对人肺癌A549细胞凋亡及侵袭力的影响
- Author:
Jing ZHANG
;
Yanjie SHEN
;
Hongxia MEI
;
Han LIN
;
Jun LI
;
Qingquan LIAN
;
Wangning SHANGGUAN
- Publication Type:Journal Article
- Keywords:
Propofol;
Apoptosis;
Neoplasm invasiveness
- From:
Chinese Journal of Anesthesiology
2014;34(9):1079-1081
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of propofol on apoptosis and invasiveness of human lung cancer cell line A549 cells.Methods Human lung cancer cell line A549 were seeded onto 96-well plates (100 μl/well) and 6-well plates (2 000 μl/well) at a density of 2× 105 cells/ml,and cultured for24 h at 37 ℃ in 5％ CO2.The cells were randomly divided into 2 groups (n =60 each) using a random number table:dimethyl sulfoxide (DMSO) group and propofol group (group P).In group P,propofol with the final concentration of 100 μmoYL was added.In group DMSO,0.5％ DMSO with the final concentration of 0.5％ was added.At 24 h of incubation with drugs,caspase-3 expression was detected by high content screening (HCS); the expression of matrix metalloproteinase (MMP-2) was detected by Western blot analysis.At 0.5,1 and 5 h of incubation,ERK1/2 expression was also measured using Western blot analysis.Results Compared with group DMSO,the expression of caspase-3 was up-regulated,the expression of MMP-2 was down-regulated,ERK1/2 expression was up-regulated at 0.5 of incubation and down-regulated at 1 h of incubation,and no significant change was found in ERK1/2 expression at 5 h of incubation in group P.Conclusion Propofol can promote apoptosis in A549 cells and inhibit invasiveness of human lung cancer cell line A549 cells.
