Clinical implications of rearrangements during transfection of papillary thyroid cancer (RET/PTC) 1,3 detected with FQ-PCR in fine-needle aspiration specimen of thyroid nodules
10.3760/cma.j.issn.1000-6699.2014.10.003
- VernacularTitle:荧光定量PCR检测甲状腺结节针吸细胞甲状腺乳头状癌的原癌基因1,3的重排
- Author:
Lingying YU
;
Lizhen MA
;
Qiaofeng TU
;
Yueming CHEN
;
Yi ZHANG
;
Jianhua FANG
- Publication Type:Journal Article
- Keywords:
Papillary thyroid carcinoma;
RET/PTC rearrangement;
Fine needle aspiration;
Polymerase chain reaction
- From:
Chinese Journal of Endocrinology and Metabolism
2014;30(10):830-833
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical significance of rearrangements during transfection of papillary thyroid cancer (RET/PTC) 1,3 in fine needle aspiration (FNA) specimen from regional thyroid nodules by FQ-PCR.Methods Two hundred and eighty-five FNA samples were collected from patients with thyroid nodules during January 2012 to January 2013.RET/PTC1,3 rearrangements were detected with FQ-PCR.Results The frequencies of the RET/PTC1 and RET/PTC3 rearrangements in 285 FNA samples were 17.2％ (49/285) and 1.4％ (4/285),respectively.During 21.7 months of follow-up,19 (40.4％,19/47) RET/PTC1 positive patients were confirmed to have papillary thyroid carcinoma(PTC) after operation.In the patients with RET/PTC1 rearrangement PTC was found in Thy5 and Thy4 groups.In Thy 2 group,22.6％ cases with RET/PTC1 rearrangements developed PTC as compared with 3.2％ cases without it(x2 =6.667,P＜0.01).In addition,8.5％ (4/47) RET/PTC1 rearrangements emerged in benign lesions.Conclusions It is convenient and reliable to detect RET/PTC1,3 rearrangements by FQ-PCR using FNA samples.RET/PTC1 rearrangement frequently occurs in PTC,however it would be detected in benign lesions occasionally.
