Effect of serum of rats with hepato-pulmonary syndrome on expression of caveolin-1 and VE-cadherin in pulmonary microvascular endothelial cells
10.3760/cma.j.issn.0254-1416.2014.08.025
- VernacularTitle:肝肺综合征大鼠血清对肺微血管内皮细胞caveolin-1及VE-cadherin蛋白表达的影响
- Author:
Biying YUAN
;
Bin YI
;
Jing ZENG
;
Lin CHEN
;
Qian CHEN
;
Jiaxiang DUAN
;
Kaizhi LU
- Publication Type:Journal Article
- Keywords:
Serum;
Hepatopulmonary syndrome;
Endothelial cells;
Caveolin 1;
Cadherins
- From:
Chinese Journal of Anesthesiology
2014;34(8):996-999
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of the serum of rats with hepato-pulmonary syndrome (HPS) on the expression of caveolin-1 and VE-cadherin in pulmonary microvascular endothelial cells (PMVECs).Methods Among the 40 healthy Sprague-Dawley rats,aged 3-4 months,weighing 220-250 g,20 rats were taken randomly for establishment the model of HPS which was produced by chronic ligation of the common bile duct,and the left 20 rats served as sham operation group.Primary PMVECs were harvested from healthy adult Sprague-Dawley rats and inoculated in ECM culture medium or on 96-well culture plate.The PMVECs of 4th-9th generation were randomly divided into 2 groups (n =36 each):control group (group C) and HPS group.In group C,the serum obtained from normal rats in sham operation group was added to PMVECs,while the serum obtained from rats with HPS was added in HPS group.The final concentration of serum was 10％.After being incubated for 12,24 and 36 h (T1-3),the expression of caveolin-1 and VE-cadherin in PMVECs was detected by Western blot,and the PMVEC adhesion rate and proliferation were determined by CKK-8 method.Results Compared with group C,the expression of caveolin-1 and VE-cadherin was significantly down-regulated,the cell adhesion rate was decreased,and the proliferation of PMVECs was enhanced in HPS group.Conclusion The serum of rats with HPS induces weakened PMVEC contact inhibition through down-regulating caveolin-1 and VE-cadherin expression.
