Effects of propofol post-conditioning on hippocampal neuronal K+-Cl-co-transporter 2 expression in rats with cerebral ischemia/reperfusion injury
10.3760/cma.j.issn.0254-1416.2014.10.006
- VernacularTitle:异丙酚后处理对脑缺血再灌注损伤大鼠海马神经元K+-Cl-共转运体2表达的影响
- Author:
Hongbai WANG
;
Haiyun WANG
;
Guolin WANG
;
Ai ZHU
;
Shuying LIU
- Publication Type:Journal Article
- Keywords:
Propofol;
Sodium-potassium-chloride symporters;
Hippocampus;
Neurons;
Reperfusion injury;
Brain;
Postconditioning
- From:
Chinese Journal of Anesthesiology
2014;34(10):1181-1184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of propofol post-conditioning on hippocampal neuronal K+-Cl-co-transporter 2 (KCC2) expression in the rats with cerebral ischemia/reperfusion (I/R) injury.Methods Seventy-two male Sprague-Dawley rats,aged 7-8 weeks,weighing 250-280 g,were randomly divided into 3 groups (n =24 each):sham operation group (group S),cerebral I/R (group I/R) and propofol post-conditioning group (group PP).The model of focal cerebral I/R injury was established by occlusion of the right middle cerebral artery.Propofol 20 mg· kg-1 · h-1 was infused over 2 h starting from the onset of reperfusion through the femoral vein in group PP.The equal volume of normal saline was given in S and I/R groups.Modified neurological severity score (mNSS) was used to evaluate the impairment of neurological function.The animals were then sacrificed and brains were removed for determination of the number of neurons (by Nissl' s staining) and expression of KCC2 (by immunofluorescence and Western blot) in hippocampal CA3 region.Results Compared with group S,the scores of mNSS were significantly increased,and the number of neurons and expression of KCC2 in hippocampal CA3 region were decreased in I/R group,and mNSS scores were increased,and no significant changes were found in the other parameters in group PP.Compared with group I/R,the mNSS scores were significantly decreased,and the number of neurons and expression of KCC2 in hippocampal CA3 region were increased in group PP.Conclusion The mechanism by which propofol post-conditioning reduces cerebral I/R injury is related to up-regulated expression of hippocampal KCC2 in rats.
