Effect of curcumin preconditioning on activation of mast cells during intestinal ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2014.09.025
- VernacularTitle:姜黄素预处理对大鼠肠缺血再灌注时肥大细胞活化的影响
- Author:
Weicheng ZHAO
;
Chengxiang YANG
;
Hanbing WANG
;
Hongzhen LIU
;
Jing WANG
;
Bin ZHANG
;
Meijuan LIAO
- Publication Type:Journal Article
- Keywords:
Curcumin;
Reperfusion Injury;
Intestines;
Mast Cell
- From:
Chinese Journal of Anesthesiology
2014;34(9):1120-1122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of curcumin preconditioning on the activation of mast cells during intestinal ischemia/reperfusion (I/R) in rats.Methods Twenty-four female Sprague-Dawley rats,weighing 180-220 g,were randomly divided into 3 groups (n =8 each) using a random number table:sham operation group (S group),intestinal I/R group (I/R group),and curcumin preconditioning group (Cur group).Intestinal I/R was produced by occlusion of superior mesenteric artery (SMA) for 75 min followed by reperfusion.At 5 days before I/ R,curcumin 200 mg/kg (in 20 mg/ml normal saline) was given through a gastric tube in Cur group,while the equal volume of normal saline was given instead in S and I/R groups.All the rats were sacrificed at 4 h of reperfusion,and the intestinal specimens were obtained for microscopic examination and for determination of tryptase expression and β-hexosaminidase content.Intestinal damage was assessed and scored according to Chiu.Results Compared with S group,Chiu's score and β-hexosaminidase content were significantly increased,and the expression of tryptase was up-regulated in I/R and Cur groups.Compared with I/R group,Chiu' s score and β-hexosaminidase content were significantly decreased,and the expression of tryptase was down-regulated in Cur group.Conclusion The mechanism by which curcumin preconditioning attenuates intestinal I/R injury is related to inhibited activation of mast cells of rats.
