Effects of mechanical stretch preconditioning on pathological stretch-induced damage to type Ⅱ alveolar epithelial cells
10.3760/cma.j.issn.0254-1416.2014.09.021
- VernacularTitle:机械牵张预处理对机械通气性牵张致肺泡Ⅱ型上皮细胞损伤的影响
- Author:
Tao HUANG
;
Ju GAO
- Publication Type:Journal Article
- Keywords:
Biomechanics;
Ischemic preconditioning;
Epithelial cells;
Pulmonary alveoli
- From:
Chinese Journal of Anesthesiology
2014;34(9):1105-1107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of mechanical stretch preconditioning on pathological stretch-induced damage to type Ⅱ alveolar epithelial cells.Methods A549 cells cultured in vitro were randomly divided into 6 groups (n =5 each)using a random number table:control group (Ⅰ group),pathological stretch group (Ⅱ group) and different time mechanical stretch preconditioning + pathological stretch group (group Ⅲ-Ⅵ).A549 cells were exposed to 20％ cyclic stretch at 0.3 Hz for 6 h in Ⅱ group.In Ⅲ-Ⅵ groups,A549 cells were exposed to 5％ cyclic stretch at 0.3 Hz for 15,30,60 and 120 min,respectively,and then exposed to 20％ cyclic stretch at 0.3 Hz for 6 h.After the end of pathological stretch,the apoptosis in cells was detected using flow cytometry and was calculated.F-actin expression was determined using flow cytometry.Results Compared with group Ⅰ,the apoptosis rate was significantly increased,and the expression of F-actin was up-regulated in Ⅱ group.Compared with group Ⅱ,the apoptosis rate was significantly increased in groups Ⅳ-Ⅵ,and the expression of F-actin was up-regulated in groups Ⅴ and Ⅵ.Conclusion Preconditioning with mechanical stretch (30-120 min) can mitigate pathological stretch-induced damage to type Ⅱ alveolar epithelial cells.
