Release of L-EK in cerebrospinal fluid induced by intrathecal HEK293 cells modified with human preproenkephalin gene and the analgesic efficacy of L-EK for bone cancer pain
10.3760/cma.j.issn.0254-1416.2014.08.019
- VernacularTitle:鞘内注射人前脑啡肽基因修饰HEK293细胞在脑脊液释放L-EK情况及L-EK对骨癌痛的镇痛效果
- Author:
Yufei PAN
;
Baozhong YANG
;
Zhaoxia XUE
;
Feng BAI
;
Bo HU
;
Xuli GUO
- Publication Type:Journal Article
- Keywords:
Cancer pain;
Cell analgesia;
Intrathecal puncture;
Gene-modified;
Determination of pain
- From:
Chinese Journal of Anesthesiology
2014;34(8):976-978
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the release of leucine-enkephalin (L-EK) in the cerebrospinal fluid induced by intrathecal human embryonic kidney (HEK293) cells modified with human preproenkephalin (hPPE)gene and the analgesic efficacy of L-EK for bone cancer pain.Methods Forty CIBP female Sprague-Dawley rats were randomized into transplantation (CIBP+ hPPE/HEK293,n =20) and control (CIBP + HEK293,n =20)groups using a random number table.At 1 day before inoculation of cancer cells (T1,baseline) and 8,15,21,25,32 and 35 days after inoculation (T2-7),thermal paw withdrawal latency (TWL) was measured,and the number of licking/biting the claw on the transplantated side and degree of hindlimb limping during free activities were recorded.After observation at T4,10 rats were chosen from each group and sacrificed and the cerebrospinal fluid of rats was collected in an ice bath for detection of hPPE expression using radioimmunoassay.Results Compared with control group,TWL was significantly prolonged,the concentration of L-EK in the cerebrospinal fluid was increased,and the number of licking/biting the claw on the transplantated side and degree of hindlimb limping during free activities were decreased at T4-7 in transplantation group.Conclusion Intrathecal HEK293 cells modified with hPPE gene can continuously secrete L-EK and mitigate bone cancer pain.
