Effect of ginsenoside Rg1 on oligomeric Aβ (1-42) inhibition of PKA/CREB pathway
10.3760/cma.j.issn.0254-9026.2015.03.023
- VernacularTitle:人参皂苷Rg1对寡聚肽淀粉样β蛋白抑制蛋白激酶A通路的影响
- Author:
Tianwen HUANG
;
Raoli HE
;
Meng ZHOU
;
Jing ZHANG
;
Xiaochun CHEN
- Publication Type:Journal Article
- Keywords:
Ginsenoside;
Amyloid beta-protein;
Protein kinases
- From:
Chinese Journal of Geriatrics
2015;34(3):316-318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible effect of ginsenoside Rg1 and oligo Aβ1-42 on PKA/CREB pathway.Methods The damage was induced by oligomeric Aβ1-42 in primary cortical neuron.Neurons were incubated with or without glutamate,or incubated in Aβ,or pre-incubated in Rg1 and then co-incubated in Aβ.The proteins of p-CREB,t-CREB,PKA Ⅱ α and BDNF were detected by Western blot.Results After the treatment with Oligo Aβ1-42 for 2 h,the p-CREB/t-CREB level induced by glutamate was obviously lower (P＜ 0.001).However,in neurons pre incubatedwith 2.5,5.0,10.0 μmol/L of ginsenoside Rg1 and then co-incubated with 5μmol/L of oligo Aβ1-42,the p-CREB/t-CREB induced by glutamate was significantly increased as compared with that of Aβ1-42 group (P＜0.05).Upon Aβ1-42 exposure for 2 h,cortical neurons showed a statistically significant increase in PKA Ⅱ α as compared to the control group (P ＜ 0.001).Pre-treatmentwith varying doses of ginsenoside Rg1 (2.5,5,10μmol/L) showed a decrease in PKA Ⅱ α as compared to neurons treated with Aβ1-42 alone for 2 h (P＜0.001).Furthermore,BDNF level significantly increased in Rgl-pretreated cells as compared to cells treated with Aβ1-42 alone for 24h (P＜0.05).Conclusions Ginsenoside Rg1 attenuates the oligo Aβ142 inhibition of PKA/CREB pathway.
