Optimization for cell lysate preparation of M. leprae from infected nude mouse.
- Author:
Tae Jin KANG
1
;
Seong Beom LEE
;
Gue Tae CHAE
Author Information
1. nstitute of Hansen's Disease, Department of Pathology College of Medicine, The Catholic University of Korea, Korea.
- Publication Type:Original Article
- MeSH:
Animals;
Bacteria;
Electrophoresis, Polyacrylamide Gel;
Genome Size;
Leprosy;
Mice;
Mice, Nude*;
Sonication
- From:Korean Leprosy Bulletin
2001;34(1):47-56
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The method of cell lysate preparation of M. leprae is an important technique in the study of leprosy. This report describes the optimization of method for cell lysate preparation of M. leprae obtained from infected nude mouse. After M. leprae isolated from nude mouse foot-pad was disrupted by sonication, it was centrifuged and then whole lysate was prepared. With this method it was possible to isolate 0.3 mg whole cell lysate using 20 mg of M. leprae. By SDS-PAGE and Coomassie blue staining, the number of protein in M. leprae is less than that of other bacteria, for example, E. coli and M. smegmatis. It is likely that this is due to the small genome size. This work will contribute to the analysis of new protein antigen of M. leprae and the basic study for the development of vaccine in leprosy.
