The effect of tumor metastasis associated gene 1 on the process of epithelial-mesenchymal transition in esophageal squamous cell carcinoma
10.3760/cma.j.issn.1008-6315.2015.06.006
- VernacularTitle:肿瘤转移相关基因1在食管癌细胞上皮间质转化过程中的促进作用
- Author:
Huan LIU
;
Haijuan WANG
;
Chunxiao LI
;
Hui LI
;
Chen LIN
;
Haili QIAN
- Publication Type:Journal Article
- Keywords:
Metastasis associated gene 1;
Esophageal cancer;
Epitheial mesenchymal transition
- From:
Clinical Medicine of China
2015;31(6):498-502
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of metastasis associated gene 1 (MTA1) on epithelial-mesenchymal transition(EMT) of esophageal squamous cell carcinoma(ESCC).Methods Lentivirus infection method was used to establish the MTA1 knocking out cell line (LV3-shMTA1-KYSE410) and the MTA1 overexpressing cell line (LVS-MTA1-KYSE450).Western Blot was used to measure the expression of MTA1 and the proteins associated with EMT process.Furthermore,the expression and localization of E-cadherin and Vimentin were observed by immunofluorescence assay under confocal microscope.Finally,the wound healing method was performed to confirm the changes of migration ability of the established cell lines.Results When KYSE-450 cells were overexpressed MTA1,the expression level of E-cadherin was down-regulated while Vimentin was up-regulated,and the migration ability was enhanced (0.91 ± 0.00 vs.0.23 ± 0.04,P ＜0.05).When MTA1 was knocked out in KYSE-410 cells,the results were the opposite (0.19±0.01 vs 0.53±0.01,P ＜0.05).Conclusion Overexpression of MTA1 may promote the process of epithelial-mesenchymal transition and enhance the migration ability of ESCC.
