Effects of IL-10 gene therapy on TAA-induced liver fibrosis in mice and its mechanism
10.3760/cma.j.issn.1008-1372.2015.05.021
- VernacularTitle:白介素-10对硫代乙酰胺诱发小鼠肝纤维化的效果及其机制
- Author:
Zhiyong LIU
;
Jian YI
;
Xiaoming ZOU
- Publication Type:Journal Article
- Keywords:
Interleukin-10/PD/ME;
Gene therapy;
Liver cirrhosis/DT;
Thioacetamide/AE
- From:
Journal of Chinese Physician
2015;17(5):714-718
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the interleukin-10 (IL-10) gene therapy has the effect of anti liver fibrosis in mice and its mechanism.Methods Liver fibrosis was induced by long-term thioacetamide administration in mice.Human IL-10 expression plasmid was delivered via electroporation after liver fibrosis was established.The immunohistochemistry was used to study the expression of IL-10 in liver.Sircol collagen determination method was used to detect the contents of collagen in the liver.The reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expressions of liver fibrosis-related genes,including transforming growth factor-β1 (TGF-β1),collagen α1,tumor necrosis factor-α (TNF-α),intercellular adhesion molecule-1 (ICAM-1),fibronectin,vascular cell adhesion molecule 1 (VCAM-1),and matrix metalloproteinase-inhibiting factor (TIMP-1,TIMP-2).Results Immunohistochemical results showed IL-10 gene therapy reversed hepatic fibrosis.Sircol collagen assay showed that IL-10 gene therapy reduced the content of collagen fibers(P ＜ 0.05).RT-PCR revealed IL-10 gene therapy reduced liver TGF-β1,TNF-α,collagen α1,cell adhesion molecule,and TIMPs mRNA upregulation.Conclusions Electroporative IL-10 gene therapy might be an effective therapeutic reagent for liver fibrosis with potential future clinical applications.
