Tetrandrine affects the expression of DNMT in HL-60 cells
10.3760/cma.j.issn.1008-1372.2015.04.017
- VernacularTitle:汉防己甲素对HL-60细胞DNA甲基转移酶表达的影响
- Author:
Yafang GUO
;
Liangming MA
;
Yujin LU
;
Bo BAI
- Publication Type:Journal Article
- Keywords:
TETRANDRINE/AD;
HL-60 cells/DE/ME;
DNA (cytosine-5-)-methyltransferase/ME
- From:
Journal of Chinese Physician
2015;17(4):541-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of DNA methyltransferase (DNMT) in HL-60 cells induced by tetrandrine (Tet).Methods HL-60 cells were treated with different concentrations of Tet and decitabine (DAC) alone and in combination with both.Methyl thiazolyl tetrazolium (MTT) assay was used to assess cytotoxic effect.Flow cytometry (FCM) was used to determine apoptosis rate.Real-time quantitative polymerase chain reaction (PCR) assay was used to quantify mRNA levels of DNMT.Western blot was used to quantify the expression of DNMT protein.Results Tet inhibited the growth and proliferation of HL-60 cells in a time-and dose-dependent manners (both P ＜0.01).Tet treated HL-60 cells after 48 h at the concentration of 2 μmol/L,and 4 μmol/L,the levels of DNMT gene and protein in the drug administration group decreased compared to the control group.After incubation for 48 h with Tet 2 μmol/L combined with DAC 4 μmol/L,the combination group was significantly depressed.Conclusions Tet could potently inhibit the growth and proliferation of HL-60 cells,reduce the expression levels of DNMT mRNA and protein,and have a more obvious effect in the combination group.
