Effect of Rho kinase inhibitor Y27632 on the cytoskeleton of airway smooth muscle in young asthmatic rats with airway remodeling
10.3760/cma.j.issn.1008-1372.2015.04.013
- VernacularTitle:Y27632对哮喘气道重塑幼年大鼠气管平滑肌细胞骨架的影响
- Author:
Bing WEI
;
Yali LIU
;
Yunxiao SHANG
;
Yinping LI
;
Chao ZHANG
- Publication Type:Journal Article
- Keywords:
rho-associated kinases/AI/ME;
Asthma/PP/DT;
Trachea/PP/DE/ME;
Myocytes,smooth muscle/PA/DE/ME
- From:
Journal of Chinese Physician
2015;17(4):524-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alteration of the cytoskeleton of airway smooth muscle cells in young asthmatic rats with airway remodeling and the effect of RhoA/ROCK signal pathway.Methods Airway smooth muscle cells (ASMCs) were primary cultured and purified from Sprague-Dawley(SD) rats that were induced by ovalbumin (OVA) inhalation for 8w,then incubated by Pho kinase inhibitor Y27632.Real time quantitative polymerase chain reaction (RT-PCR),Western blot,and immunohistochemistry were used to measure the alteration of F-actin,and α-tubulin in the cytoskeleton of airway smooth muscle.Results (1) The asthma group showed a high average gray value of F-actin in ASMC than control groups,especially 8 weeks;and were significantly down in the group after adding Y27632(P ＜0.01).(2) The intension and intensity of fluorescence signal of α-tubulin in asthma groups in 8 weeks were higher than control greup(P ＜0.01),and were significantly decreased in Y27632 group.(3) A higher expression of α-tubulin protein was shown in the asthma group in 8 weeks relative to control group(P ＜0.01),and was significantly down-regulated in Y27632 group(P ＜0.05).Conclusions Alteration of the cytoskeleton of airway smooth muscle exists in young asthmatic rats and the RhoA/ROCK signal pathway possibly plays a significant role.
