Study on the therapeutic effects of hyperbaric oxygen on human umbilical cord Wharton jelly mesenchyme cells on myocardial infarction rats
10.3760/cma.j.issn.1008-1372.2015.07.017
- VernacularTitle:高压氧对人脐带华通胶间充质干细胞移植治疗大鼠心肌梗死的效应性研究
- Author:
Hong ZHANG
;
Xuesheng LUO
;
Gongda JIANG
;
Xiao CHEN
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygenation;
Mesenchymal stem cell transplantation;
Myocardial infarction/SU;
Umbilical cord/CY/SU
- From:
Journal of Chinese Physician
2015;17(7):1013-1016
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether hyperbaric oxygen could enhance the effects of the transplanted stem cell on treating myocardial infarction rats.Methods A total of 70 Sprague-Dawley (SD) Rats was randomly divided into four groups,sham operated,acute myocardial infarction (AMI),mesenchymal stem cells (MSCs) (Stem Cell Transplanting),and hyperbaric oxygen (HBO) + MSCs group,respectively.Myocardial infarction rat model was established,hyperbaric oxygen treatment was carried out every day at the first to 30 day after AMI.Human umbilical cord Wharton jelly mesenchyme cell was transplanted at the third day after AMI.The densities of polymorphonuclear neutrophils and macrophages in the infracted border zones and in the infarcted area of heart were determined with hematoxylin and eosin (HE) staining.The cardiac function was assessed with M-mode transthoracic echocardiography,in vivo.Results (1)Compared to MSCs group,the stem cell proliferation and survival rates were increased significantly at the forth week (276.6 ±73.8 versus 20.5 ± 12.3,P ＜0.01).(2)Compared to the AMI and MSCs groups,the infracted area and the number of inflammatory cell infiltration were significantly reduced in HBO alone and MSCs + HBO groups.(3) Compared to the AMI group,the cardiac function was significantly improved at the different degree in the other three groups;among these groups,cardiac function was significantly improved in the MSCs + HBO group (P ＜0.01),except for the volume of end-dilation (P ＞0.05).Conclusions Hyperbaric oxygenation could significantly promote the proliferation and survival rate of stem cells,and improve the cardiac function.Thus further enhance the effect of the transplanted stem cell on treating the infarcted heart.
