Bioinformatics analysis of microRNAs differently expressed in major depression disorder
10.3760/cma.j.issn.1674-6554.2015.05.009
- VernacularTitle:重症抑郁障碍外周血microRNA的生物信息学分析
- Author:
Huimin FAN
;
Wenbo WU
;
Wei NIU
;
Xinyang SUN
;
Aifang ZHONG
;
Lin ZHAO
;
Liyi ZHANG
- Publication Type:Journal Article
- Keywords:
MiRNA;
Major depressive disorder;
Target gene;
Bioinformatics
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2015;24(5):418-422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To predict the target genes and function of has-miR-26b,has-miR-1972,has-miR4485,has-miR-4498,and has-miR-4743 by bioinformatics analysis,and provide the theoretical basis for the further research.Methods The targets of the five microRNAs were predicted by Target Scan,miRBD,and DIANA-microT-CDS,and the result were analyzed by gene ontology and pathway analysis using FunNet.Results 734 predicted targets were obtained by finding the intersected genes of Target Scan,miRBD,and DIANA-microT-CDS.GO analysis showed that biological processes regulated by the differentially expressed microRNAs included diverse terms,among which some terms (e.g.,central nervous system development,neuron differentiation,axonogenesis,synaptic transmission,learning,and memory,etc.) had direct relationship with the central nervous system and brain functions.The pathway analysis showed that a significant enrichment in several pathways related to neuronal brain function,such as axon guidance,glutamatergic synapse,Wnt signaling pathway,mTOR signaling pathway,VEGF signaling pathway,etc.Among the five microRNAs,has-miR-26b,has-miR-1972,has-miR-4498 might have more important regulatory functions.Conclusion Bioinformatic analysis indicates that has-miR-26b,has-miR-1972,has-miR-4485,has-miR-4498,and has-miR-4743 are closely related to the mechanism and pathogenesis of major depressive disorder.
