Protective effects of simvastatin on injured dopaminergic neurons in Parkinson's disease rats induced by lipopolysaccharide
10.3760/cma.j.issn.1674-6554.2015.03.001
- VernacularTitle:辛伐他汀对脂多糖诱导帕金森病大鼠多巴胺神经元损伤的保护作用
- Author:
Tan WANG
;
Beisha TANG
;
Xuebing CAO
;
Zhibin CHEN
;
Xiaowu CHEN
;
Peipei HUANG
;
Tian ZHANG
- Publication Type:Journal Article
- Keywords:
Parkinson's disease;
Simvastatin;
Lipopolysaccharide;
Glial cells
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2015;24(3):193-197
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effect of simvastatin treatment on Parkinson's disease rats induced by lipopolysaccharide (LPS) and its mechanism.Methods The LPS-PD model was established by injection of LPS (5 mg/mL) into the right substantia nigra compacta (SNC),and rats were randomly divided into control group,LPS-model group and simvastatin treatment group with 15 rats in each group.Rats in the simvastatin treatment group was intraperitoneally administered simvastatin (5 mg/kg) before,and daily for 14 days after surgery,while the control group and LPS-model group received same volume normal saline and LPS respectively.Ionized calcium binding adaptor molecule 1 (Iba-1)-positive cells and the expression of tyrosine hydroxylase (TH),tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the SNC were detected by immunohistochemistry,Western blotting and enzyme-linked immunosorbent assay,respectively.The effect of simvastatin in the PD model was also examined in behavioral tests.Results The LPS-model group exhibited typical animal PD behaviors.Compared with the control group,the LPS-model group exhibited a decreased number of DA neurons,and comparison of the intact side to reduce 81.13％ (P＜0.01) in the SNC,as well as increases in the Iba-1-positive cell number,iNOS,IL-1β and TNF-α expression (P＜0.05).These effects were inhibited by simvastatin treatment (P＜0.05).Conclusion Simvastatin mediates a protective effect on dopaminergic neurons in the SNC in the LPS-PD model,possibly by inhibiting glial cells (astrocytes and microglia) activation,and playing an anti-inflammatory role,thus improving substantia nigra function.
