The protective effects of ischemic postconditioning on lung ischemic reperfusion injury in rats via PI3K/Akt pathway
10.3760/cma.j.issn.1001-4497.2014.10.016
- VernacularTitle:缺血后处理对鼠在体肺缺血再灌注损伤的保护机制
- Author:
Yao FU
;
Junbin LAN
;
Mingke DUAN
;
Yigen WU
;
Shengshui XU
;
Yukun BAI
- Publication Type:Journal Article
- Keywords:
Rats,sprague-dawley;
Lung;
Reperfusion injury;
1-Phosphatidylinositol 3-kinase
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2014;30(10):628-631
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of ischemic postconditioning on lung injury in situ during lung ischemic reperfusion.Methods 24 SD rats were randomly divided into 3 groups,sham-operated group(S),ischemic-reperfusion group(I/R) and ischemic postconditioning group (IpostC).IpostC was established by several brief reperfusion-ischemia (30 s,5 cycles) before continuous reperfusion.AKT,p-AKT,p70S6K,p-p70S6K protein expression,apoptotic cells were tested by Western blotting and TUNEL,wet to dry weight ratio(W/D) in lung tissue were determined respectively.The lung pathological changes were also observed.Results Compared with S group,expression of Akt、p-Akt、p70S6K、p-p70S6K and the ratio of W/D,apoptotic index in lung tissue all markedly increased in I/R and IpostC group (P ＜ 0.05).Compared with I/R group,expression of Akt、p-Akt、p70S6K、p-p70S6K markedly increased in IpostC group(P ＜0.05),while the ratio of W/D and apoptotic index significantly reduced (P ＜ 0.05),the pathological injury in IpostC group also reduced significantly.Conclusion IpostC has a protective effect on lung ischemic reperfusion injury via PI3K/Akt pathway.
