The comparison between the effect of sufentanil and morphine to the ischemia-reperfusion myocardial Cx43 and ventricular arrhythmias in rats
10.3760/cma.j.issn.1001-4497.2014.10.013
- VernacularTitle:舒芬太尼和吗啡对缺血再灌注大鼠心肌Cx43及室性心律失常影响的比较
- Author:
Bing ZHANG
;
Dongmei ZHANG
;
Yun WANG
;
Jianzhen WANG
;
Haifeng JIANG
;
Xuan ZHANG
;
Xiangzhao XU
- Publication Type:Journal Article
- Keywords:
Sufentanil;
Morphine;
Myocardial reperfusion injury;
Connexin 43
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2014;30(10):618-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective The study was to compare the effect of sufentanil and morphine preconditioning on ischemia /reperfusion-induced ventricular arrhythmias and the expression and distribution of myocardial Cx43 in rats.The regulation mechanisms that how sufentail and morphine lead to the change of Cx43 were also studied.Methods 32 male SD rats were randomly divided into 4 groups:sham operation group (group C),ischemia/reperfusion (group I/R),morphine preconditioning group(group M) and sufentanil preconditioning group(group S),each group had 8 rats.Established myocardial ischemia/reperfusion model,continuous recorded Ⅱ ECG,mean arterial pressure(MAP) and heart rate(HR).The ventricular arrhythmias at the 30 min before reperfusion was observed and the ventricular arrhythmias score of each group was calculated by ECG analysis; expression and distribution of Cx43 protein were observed by immunohistochemical technique.Results Compared with group C,the HR,MAP,RPP of group I/R were decreased obviously (P ＜ 0.05),while the arrhythmia score was significantly higher(P ＜ 0.05).Compared with group I/R,the extent of the declined of HR,MAP,RPP of group M and group S were eased significantly(P ＜ 0.05) and arrhythmia score was significantly lower(P ＜ 0.05).The HR,MAP,RPP of group M and group S are closer(P ＞ 0.05).Compared with the group C,Cx43 expression level in group I/R was significantly reduced (P ＜ 0.05) and the distribution was disordered,while compared with the group I/R,Cx43 expression level in group M and group S were significantly increased (P ＜ 0.05),and its distribution was structured.In group M and group S,Cx43 expression level were closer(P ＞ 0.05) and so as their distribution.Conclusion Sufentanil and morphine could inhibit the reduction of myocardial Cx43 expression level and improve its distribution which could played an important role in anti-arrhythmic during ischemia-reperfusion.
