Radiography evaluation of enhanced vascularization of COMP-Ang-1 on acellular nerve
10.3760/cma.j.issn.1001-2036.2015.01.014
- VernacularTitle:COMP-Ang-1促去细胞神经血管化的放射显影法评价
- Author:
Longhai QIU
;
Bo HE
;
Zhaowei ZHU
;
Jun HU
;
Xiaolin LIU
;
Jiakai ZHU
- Publication Type:Journal Article
- Keywords:
Acellular nerve;
COMP-Ang-1;
Vascularization;
Radiography;
Nerve repairing
- From:
Chinese Journal of Microsurgery
2015;38(1):56-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the way of vascularization of acellular nerves and evaluate the enhanced vascularization of using COMP-Ang-1 into acellular nerve on bridging sciatic nerve gaps by radiography.Methods From March,2013 to June,2014,acellular nerves were harvested by chemical extraction.Thirty-six female rats weighing 200-250 g were randomly divided into 2 groups:18 animals with 1 cm long sciatic nerve lesions were repaired by nerve grafting (control group),18 animals with 1 cm long sciatic nerve lesions were repaired by nerve grafting and COMP-Ang-1 were administrated after surgery.Grafts were harvested after perfusion of lead oxide (carotid artery) on day 7,day 14 and day 21 postoperatively.Radiography was performed to capture the two dimensional image.The rules of vascularization of acellular nerve and the enhanced effects of COMP-Ang-1 on vascularization were evaluated.Results The density of vessels in COMP-Ang-1 group were higher than control group after 7 days (2701.60 ± 318.93 vs.925.40 ± 106.22,P =0.030),14 days (3309.21 ± 381.31 vs.2832.70 ± 189.23,P =0.210) and 21 days (4787.33 ± 251.09 vs.3469.36 ± 232.10,P =0.030) postoperatively; the area of vessels in COMP-Ang-1 group were higher than control group after 7 days (9231.03 ± 581.91 μm2 vs.4839.01 ± 101.01 μm2,P =0.043) and 14 days (15561.13 ± 697.73 vs.6811.07 ± 250.05,P =0.049) postoperatively.Conclusion COMP-Ang-1 can enhance the vascularization of acellular nerves fairly.
