Establishment of luciferase-labeled mesenchymal stem cells

VernacularTitle:荧光素酶基因稳定表达的间充质干细胞系的构建
Author: Yuan DONG ; Caixia LI ; Xian ZHAO ; Liu LIU
Publication Type:Journal Article
Keywords: Mesenchymal stem cells; Luciferase; Lentiviral; Transfection
From: Chinese Journal of Microsurgery 2014;(6):569-572
CountryChina
Language:Chinese
Abstract: Objective To establish luciferase-labeled mesenchymal stem cells in vitro.Methods From May,2013 to January,2014,recombinant lentiviral vectors containing luciferase gene was transfected in to mesenchymal stem cell line C3H10T1/2.Puromycin was used to filter the cells.Western-blot and immunofluorescenceimaging were used to detect the transfection efficiency.Results Western blotting analysis showed that the size of this protein in cells expressing about 60KD.Showed that infected C3H10T1/2 cells expressing luciferase protein.I mmunofluorescence,compared with the uninfected control group,virus-infected cells group of about 100％ of the cells were positive for red fluorescence.Show that had built a good stable expression of luciferase C3H10T1/2 cells.Conclusion A monoclonal cell line stably and highly expressing luciferase is obtained with luciferase-labeled mesen chymal stem cells.