The effects of stromal cel1-derived factor 1 on the migration of neural stem cells and the recovery of lower limb function after spinal cord injury
10.3760/cma.j.issn.0254-1424.2015.06.003
- VernacularTitle:基质细胞衍生因子-1对神经干细胞移植治疗脊髓损伤大鼠疗效的影响
- Author:
Zhe LI
;
Ganghua GUO
;
Guosheng WANG
;
Qiuzhen ZHANG
;
Chenxia GUAN
;
Lin YUE
- Publication Type:Journal Article
- Keywords:
Neural stem cells;
Spinal cord injury;
Stromal cel1 derived factor
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2015;37(6):411-415
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effect of stromal cell-derived factor 1 (SDF-1) on the migration of neural stem cells and recovery of lower limb function after spinal cord injury (SCI).Methods A modified version of Allen's method was used to establish a SCI model in each of 96 adult male Sprague-Dawley rats.They were then randomly divided into group A which received an injection of phosphate buffer solution,group B which received an injection of neural stem cells,group C which received a combination of SDF-1 and neural stem cells,and group D which received AMD3100 and neural stem cells 7 days after the modeling.The functioning of the hind limbs of all of the rats was assessed on the 7th,14th,21st and 28th day after the modeling.The rats were then sacrificed and frozen sections of their spinal cords were stained with hematoxylin-eosin (HE) and marked with CM-Dil under fluorescent light.Results An accumulation of fluorescing cells were observed in spine cords from both group B and C,with the counts of group B [(23.6 ±3.7),(18.9 ±5.6)and(15.2 ±4.3) respectively] at the day 14,21 and 28 after modeling significantly smaller than those of group C [(27.4 ± 4.7),(20.4 ± 5.2) and (18.3 ± 3.9) respectively].During the same period of time,the average BBB scores of groups B and C were significantly better than those of groups A and D.Moreover,the average score of group C was significantly higher than group B's at all time points.Conclusions Transplanted neural stem cells can migrate to the injured site,surviving and differentiating to promote the recovery of limb function in rats with spinal cord injury.SDF-1 can promote that migration and proliferation.Moreover,CXCR4 receptor's antagonist AMD3100 can significantly hinder neural stem cells' migration to the injured site on the spinal cord.
