The effect of low intensity laser irradiation on the expression of transforming growth factor-β1 and interleukin-1β in scalded dermal tissues
10.3760/cma.j.issn.0254-1424.2015.03.004
- VernacularTitle:低强度激光照射对小鼠烫伤创面转化生长因子-β1和白细胞介素-1β基因表达的影响
- Author:
Qiang DUAN
;
Bingshui WANG
;
Shouqin SHAN
;
Xiang MU
;
Hua YUAN
;
Wei LIU
- Publication Type:Journal Article
- Keywords:
Lasers;
Inflammation;
Transforming growth factor beta-1;
Interleukin-1 β;
Scalds
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2015;37(3):176-178
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of low intensity laser irradiation on wound healing and the expression of transforming growth factor beta-1 (TGF-β1) and interleukin beta-1 (IL-1β) in wounded tissue.Methods Steam was used to scald 60 BALB/c mice.They were then randomly divided into a laser group and a control group with 30 in each group.The wounds in the laser group were irradiated with a low-intensity He-Ne laser,while the control group was given the same treatment except that the instrument was turned off.The wound healing rate was calculated,and the full wound thickness was measured and a reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of TGF-β1 and IL-1β right after the scalding and 1,3,7 and 14 days later.Results Seven and 14 days later,the wound healing rates in the laser group were (51.48 ± 5.89) ％ and (73.96 ± 7.25) ％,respectively.Compared with the control group at the same time points,the difference was statistically significant.In the laser group the expression of TGF-β1 had increased significantly at days 1 and 3,then increased to a maximum at day 7.It was significantly greater than in the control group at all three of those time points.At day 14 the expression of TGF-β1 in the laser group was significantly lower than in the control group.The expression of IL-1 β in the laser group had decreased significantly at days 1 and 3,and gradually reduced to a minimum at day 7.It was significantly lower than in the control group at all three of those time points.Conclusion Low intensity He-Ne laser irradiation can accelerate wound healing after scalding,at least in mice.Its effect may be related to its regulation of the expression of IL-1 β and TGF-β1.
