The protective effect of hydroxytyrosol on contrast-induced nephropathy and endoplasmic reticulum stress
10.3760/cma.j.issn.1001-7097.2015.05.012
- VernacularTitle:羟基酪醇保护造影剂诱导的肾脏损伤并抑制内质网应激
- Author:
Yan SUN
;
Wenxuan JI
;
Wenhong SHAN
;
Guimei LIU
;
Junyan HUANG
- Publication Type:Journal Article
- Keywords:
Contrast media;
Endoplasmic reticulum stress;
Caspase 12;
Glucoseregulated protein 78;
Hydroxytyrosol
- From:
Chinese Journal of Nephrology
2015;31(5):387-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of glucose-regulated protein 78(GRP78)and cysteine aspartic acid protease 12(Caspase-12) and evaluate the endoplasmic reticulum stress (ERS) in rats with contrast-induced nephropathy (CIN),and observe the protective effects of hydroxytyrosol on CIN rats.Methods Eighty-four Wistar rats,(220±20) g,were randomly divided into control group,CIN group,hydroxytyrosol treated group (group C+H).At 12th,24th,48th,72th day after the rats model were established,BUN and Scr were detected.ELISA were used to detect the expression of methane dicarboxylic aldehyde (MDA),superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px).HE staining were used to evaluate the pathological change of kidney.TUNEL were used to detect the apoptosis of tubular ceils.Real-time PCR were used to detect the expression of GRP78 mRNA and Caspase-12 mRNA in tubular cells.Immunohistochemistry and Western blotting were used to detect the expression of GRP78 and Caspase-12 protein in tubular cells.Results BUN,Scr,the mRNA and protein expression of GRP78,Caspase-12 in hydroxytyrosol treated group were higher than that in control group(P ＜ 0.05),but were significantly lower than that in CIN group (P ＜ 0.05).Pathological changes and the apoptosis of tubular cells in CIN group were more serious than that in hydroxytyrosol treated group (P ＜ 0.05).Conclusions Endoplasmic reticulum stress may be associated with contrast-induced nephropathy.Hydroxytyrosol can protect kidney from contrast medium via reducing the endoplasmic reticulum stress.
