Validation of the method of quantification of chlorpheniramine enantification of chlorpheniramine enantiomers in human plasma by using achiral-chiral HPLC
- Author:
Hiep Tung Bui
- Publication Type:Journal Article
- Keywords:
Chlorpheniramine
- MeSH:
Chlorpheniramine;
Plasma
- From:Journal of Medical and Pharmaceutical Information
2001;(10):25-28
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The enantiomers of chlopheniramine were determined separately in plasma by using a coupled achiral-chiral chromatographic system. The two enantiomers of the studied compound and the internal standard were separated from the biological matrix on a cyanopropyl column and reinjected into a chiral amylose AD column where the two enantiomers were separated and quantified by UV detection. The method was validated for chlorpheniramine within the range 0-20ng/ml.
