Effects and mechanisms of a novel chalcone analogue-H175 on inducing colon cancer cells' apoptosis

VernacularTitle:查尔酮衍生物H175促结肠癌细胞凋亡及其机制研究
Author: Yusheng SUN ; Jun FANG ; Jinji JIN ; Lijun TAO ; Zhuha ZHOU ; Guanbao ZHU
Publication Type:Journal Article
Keywords: Colonic neoplasms; Chalcons; Apoptosis
From: Chinese Journal of General Surgery 2015;30(6):466-470
CountryChina
Language:Chinese
Abstract: Objective To investigate the anti-tumor activity of a novel chalcone analogue-H175.Methods We synthesized chalcone analogues and further tested the cytotoxicity on colon cancer cell line (CT26) through MTT method,and identified H175 with powerful biological activity.Flow cytometry was used to assay the ability of H175 to induce CT26's apoptosis and inhibit its cell cycle.Western blot was used to detect the effect of H175 on ATF4 and CHOP expression in CT26.After knockdown CHOP by siRNA technology,further investigate the influence of H175 on cell's apoptosis through immunofluorescence (IF) and FCM.Results H175 inhibits the proliferation of CT26 (ICs0 =1.9 μmol/L),by inducing CT26's apoptosis inhibiting its cell cycle,on a dose-dependent manner of key protein CHOP of endocytoplasmic reticulum stress (ERS) signal path.siRNA technology confirmed H175 induce apoptosis by stimulating the CHOP over-expression.Conclusions Novel chalcone analogue-H175 show inhibitory effects on the proliferation of CT26 by activating the ERS signal path's key protein CHOP.