An experimental study on human embryonic stem cell-derived hepatocytes transplantation into liver decompensated mice

VernacularTitle:人胚胎干细胞来源的肝细胞小鼠移植的实验研究
Author: Daoming LIANG ; Yi ZHANG ; Min LUO ; Yongqing DUAN ; Lin ZHOU ; Hua WANG
Publication Type:Journal Article
Keywords: Embryonic,stem cells; Stem cell transplantation; Animal experimentation
From: Chinese Journal of General Surgery 2015;30(3):211-214
CountryChina
Language:Chinese
Abstract: Objective To study the ability of human embryonic stem cells to integrate into mouse liver and to repair chronic liver injury of the recipient.Methods On day 1,day-7 and day-15 after human embryonic stem cells were induced to differentiate into hepatocyte-like cells and were transplanted into mice with chronic liver failure,liver histopathology,liver function,liver tissue regeneration and mature hepatocytes of mice were tested respectively.Results Hepatic tissue pathology of mice significantly improved after transplantation and necrotic foci diminished,hemorrhage and congestion of hepatic cells relieved,and liver function improved.It was observed that human embryonic stem cells survived,proliferated,integrated with host liver,and differentiated into mature hepatocytes.Conclusions Human embryonic stem cells xenotransplanted into mice can participated liver tissue regeneration to some extent,and differentiated into functional liver cells.